A chemical method for the determination of oestriol, oestrone and oestradiol in human urine.

A method for the determination of oestriol, oestrone and oestradiol-17beta in human urine by partition chromatography and colorimetric estimation.

The direct radioimmunoassay of oestrogen glucuronides in human female urine.

Radioimmunoassays for five oestrogen metabolites in urine are described; they are oestrone 3-glucuronide, oestradiol 3-glucuronide, oestradiol 17 beta-glucuronide, oestriol 3-glucuronide and oestriol 16 alpha-glucuronide. These assays have proved accurate and reliable and can be performed rapidly; they have been carried out directly in diluted menstrual cycle urine and pregnancy urine. No sampl...

Circulating oestrogen concentrations during pregnancy in the African elephant (Loxodonta africana).

Oestrone, oestradiol-17 beta and oestriol were measured in plasma samples from non-pregnant and pregnant African elephants shot in the wild. Enzymic hydrolysis of plasma showed that approximately 90 and 96% of the total (i.e. conjugated plus unconjugated) concentrations of oestrone and oestradiol-17 beta, respectively were represented by conjugated hormones. Unconjugated oestrogens remained low...

The application of the Kober test to the quantitative estimation of oestrone and oestriol in human pregnancy urine.

IT is well known that both oestrone and oestriol when warmed with concentrated sulphuric acid give a green-fluorescing orange-coloured solution, thus resembling sterols and the bile acids. The colours given by the latter compounds are discharged by dilution with water, but as Kober [1931] showed, the oestrin colour on dilution with water is changed to a clear red, the green fluorescence being r...

Oestrogen Metabolism and Excretion in Liver Disease.

Numerous workers have reported a raised output of urinary oestrogens in men and women with various types of liver disease. Glass, Edmonson, and Soll (1940), Rupp, Cantarow, Rakoff, and Paschkis (1951), Pincus, Rakoff, Cohen, and Tumen (1951), and Dohan, Richardson, Bluemle, and Gyorgy (1952), using bioassay methods, all found in approximately 48% of the patients studied values which usually wer...